Recombinant human defensin beta-2 (BD-2), is a 4.3kD peptide comprised of 41 amino acid residues. BD-2, a member of the beta defensin family, is secreted at epithelial surfaces of the skin and respiratory tract and by some leukocytes. This defensin is induced by bacterial products and cytokines during inflammation and functions as part of the innate immune system, having a wide ranging antimicrobial activity. BD-2 also functions as a chemoattractant to immature dendritic cells and memory T cells and acts as a ligand for TLR4, upregulating co-stimulatory molecules and inducing dendritic cell maturation.||Source:|Recombinant protein corresponding to GIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP of human Defensin beta-2 expressed in E. coli||Molecular Weight: |~4.3kD||Applications: |Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.||Recommended Dilution:|ELISA: 0.2-0.4ng/well|Western Blot: 1.5-3.0ng/lane |Functional Assays: 10-100ng/ml|Optimal dilutions to be determined by the researcher.||Endotoxin: <0.1ng/ug ||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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