The Jo-1 antigen is histidyl-transfer ribonucleic acid (t-RNA) synthetase. This enzyme is partially responsible for attaching t-RNA to their cognate rRNA (2). The Jo-1 antigen migrates as a 53 kD protein on SDS-PAGE. ||Anti-Jo-1 autoantibodies were originally described as precipitating autoantibodies in sera of patients with polymyositis. It was later realized that the anti-Jo-1 antibodies were specific for patients with polymyositis. The target for the anti-Jo-1 antibodies was one of a family of distinct cellular enzymes, the aminoacyl t-RNA synthetases (1). ||The presence of autoantibodies against the Jo-1 antigen has been reported in up to 23% of polymyositis patients by immunodiffusion (3). Anti-Jo-1 antibodies are almost completely specific for myositis, being more common in polymyositis than dermato-myositis(4), and rare in children (5). The presence of anti-Jo-1 antibodies defines a distinct group of polymyositis patients with interstitial disease, arthritis, and fevers (6). The anti-Jo-1 response appears to be self-antigen driven, having a broad spectrotype over time undergoing isotype switching. Anti-Jo-1 antibodies also inhibit the function of human histidyl tRNA synthetase more than they do from other species (6). ||Recent developments in ELISA meology have increased the sensitivity therefore the diagnostician's ability to quantitate patient antibody titers.||Application:|Recommended for use in standard solid phase immunoassays.||Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 whenassayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions/protocols.||Storage Conditions:|Product unstable above 0°C, aliquot and store frozen at -70°C. Avoid repeated freeze-thaw.

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