Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108kD protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.||Source:|Recombinant protein corresponding to human Alanyl t-RNA Synthetase, fused to 6x his-tag, expressed in Sf9 insect cells.||Molecular Weight: |~107.6kD||Immunological Functions:|Binds IgG-type human auto-antibodies.||Applications: |Suitable for use in standard ELISA (checker-board analysis of positive/negative sera panels immuno-dot test), Western Blot (with monoclonal anti-hexa-His-tag antibody & Polymyositis sera), Biotinylation and Iodination. Other applications not tested.||Recommended Dilution:|ELISA: 0.3-0.8ug/ml (depending on the type of ELISA plate and coating buffer)|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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