A 21aa Peptide sequence near the C-terminus of human tankyrase.||The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. 4.8 kb of telomeric DNA is lost with each cell division. Telomere length is maintained by regulating the activity of telomerase. Purification of telomerase from the ciliate also revealed two protein of 43 and 123kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity) and show 20% homology. Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; ~127kD). hEST2 was localized in the nucleus. It is associated with telomerase activity and TP1. Telomeric Repeat binding Factors (TRF1 and TRF2) bind the telomeric TTAGGG repeat. TRFs have been colocalized with telomeric DNA in metaphase cells. TRFs are located at chromosome ends during metaphase. TRF2 protects chromosome ends and TRF1 regulates telomere length. Over expression of TRF1 resulted into telomere shortening, whereas inhibition of TRF1 increased telomere length.||Most recently, Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa), a protein with homology to ankyrins and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. Tankyrase binds to TRF1. It contains 24 ankyrin repeats in TRF-1 interacting domain. Tankyrase has PARP activity in vitro, with both TRF1 and tankyrase acting as acceptor for ADP-ribosylation.||Storage and Stability: |Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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