PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.||Source:|Recombinant protein corresponding to full length human phosphodiesterdase 7A1 expressed in S. frugiperda using a baculovirus expression system.||Biological Activity:|≥0.5u/ul||Unit Definition:|One unit is defined as the amount of enzyme that is required to convert 1pmol cAMP to AMP per min at 30°C, pH 7.5.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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