Serine/Threonine-proteinphosphatasePP1-alphacatalyticsubunit(PPP1CA)iscrucialforcelldivision,andparticipatesintheregulationofglycogenmetabolism,musclecontractilityandproteinsynthesis.ThePPP1CAproteinmayhaveasignificantroleindephosphorylatingsubstratessuchasthepostsynapticdensity-associatedCa2+/calmodulindependentproteinkinaseII.||Recombinantproteincorrespondingtoaa1-330fromhumanPPP1CA,fusedtoHis-tagatN-terminus,expressedinE.coli.||MolecularWeight:|~39.7kD(350aa,aa1-330+NTHis-Tag)||BIOLOGicalActivity:|SpecificActivity:>3000units/mg.Oneunitwillhydrolyze1nmoleofp-nitrophenylphosphatate(pNPP)/minuteat37°C,pH7.5using10mMofsubstrate.||AminoAcidSequence:|MGSSHHHHHHSSGLVPRGSHMSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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