GPX3,alsoknownasglutathioneperoxidase3,belongstotheglutathioneperoxidasefamily,whichfunctionsinthedetoxificationofhydrogenperoxide.GPX3protectscellsandenzymesfromoxidativedamage,bycatalyzingthereductionofhydrogenperoxide,lipidperoxidesandorganichydroperoxide,byglutathione.Thisproteinisoneofonlyafewproteinsknowninhighervertebratestocontainselenocysteine,whichoccursattheactivesiteofglutathioneperoxidaseandiscodedbythenonsense(stop)codonTGA.||Source:|Recombinantcorrespondingtoaa21-226fromhumanGPX3(U73C),fusedtoHis-tagatN-terminus,expressedinE.coli.||MolecularWeight:|~25.7kD(227aa)confirmedbyMALDI-TOF||AASequence:|MGSSHHHHHHSSGLVPRGSHMQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYCGLTGQYIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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