Argininemethyltransferasethatcanbothcatalyzetheformationofmonomethylarginine(MMA)andasymmetricaldimethylarginine(aDMA),withastrongpreferencefortheformationofaDMA.Preferentiallymethylatesarginylresiduespresentinaglycineandarginine-richdomainanddisplayspreferenceformonomethylatedsubstrates.SpecificallymediatestheasymmetricdimethylationofhistoneH3Arg-2toformH3R2me2a.H3R2me2arepresentsaspecifictagforepigenetictranscriptionalrepressionandismutuallyexclusivewithmethylationonhistoneH3Lys-4(H3K4me2andH3K4me3).IttherebyactsasatranscriptioncorepressorofvariousgenessuchasHOXA2.AlsomethylateshistoneH2AandH4Arg-3(H2AR3meandH4R3me,respectively).ActsasaregulatorofDNAbaseexcisionduringDNArepairbymediatingthemethylationofDNApolymerasebeta(POLB),leADIngtostimulatethepolymeraseactivitybyenhancingDNAbindingandprocessivity.MethylatesHMGA1.MayplayaroleininnateimmunityagainstHIV-1incaseofinfectionbymethylatingandimpairingthefunctionofvariousHIV-1proteinssuchasTat,RevandNucleocapsidproteinp7(NC).||Source:|RecombinantproteincorrespondingtohumanPRMT3,aa2-375(end),fusedtoN-terminalHis-tag,expressedinE.coli.||MolecularWeight:|~43kD||SpecificActivity:|~0.015pmol/min/ug ||AssayConditions:|50ulreactionmix(20mMphosphatebufferpH7.4,20uMS-adenosylmethionine,and1-5ngmethyltransferasePRMT6)isaddedtomicrowellscoatedwithhistonesubstrate.Incubateat30°Cfor2hr.AddantibodyagainstmethylatedArgresidueofhistoneH3,incubate1hr.Then,addsecondaryHRP-labeledantibodyandincubate30min.Finally,addHRPchemiluminescentsubstratesandreadluminesence.||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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