GlycineN-methyltransferase(GNMT)isatetramericcytosolicproteinwhichcatalyzesthetransferofamethylgroupfromS-adenosylmethionine(AdoMet)toglycineproducingS-adenosylhomocysteine(AdoHcy)andsarcosine(1).GNMTplaysamajorroleinmaintainingnormalAdoMetlevels.GNMTisabundantintheliverwhereitisamajorfolate-bindingprotein.Itbinds5-methyltetrahydrofolatepentaglutamateinvivoandinvitro,andthebindingofthefolateinhibitstheactivityofGNMT.AstudyoftheratenzymeshoweddifferencesinthekineticparametersbetweenrecombinantGNMTandtheproteinisolatedfromliver(2).Basedonstructuralinformation,theN-terminalpartoftheproteinplaysamajorroleinthetransferofthemethylgroupfromAdoMettoglycine(3).IthasbeenproposedthatthedifferenceinfolateinhibitorbindingbetweenrecombinantGNMTandtheliverenzymemaybeduetodifferentialacetylationoftheN-terminalvaline(4).HypermethioninemiaiscausedbydefectsinGNMT.||Source:|Recombinantcorrespondingtoaa1-295fromhumanGlycineN-methyltransferase/GNMT,expressedinE.coli.||MolecularWeight:|~33kD||EndotoxinLevel:|≤1EU/1ug(LAL)||BIOLOGicalActivity:|MeasuredbyitsABIlitytotransferamethylgroupfromS-adenosylmethioninetoglycine.|Thespecificactivityis>190pmoles/min/ug.||StorageandStability:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestableforatleast6months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！