Peptidyl-prolylcis-transisomerase-like3inhumansisencodedbythePPIL3gene.Thisgeneencodesamemberofthecyclophilinfamily.Cyclophilinscatalyzethecis-transisomerizationofpeptidylprolylimidebondsinoligopeptides.Theyhavebeenproposedtoacteitherascatalystsorasmolecularchaperonesinprotein-foldingevents.||Source:|Recombinantproteincorrespondingtoaa1-161fromhumanPPIL3,fusedtoHis-tagatN-terminus,expressedinE.coli.||MolecularWeight:|~20.3kD(181aa,aa1-161+NTHisTag)||SpecificActivity:|>280nmoles/min/mg,definedastheamountofenzymethatcleaves1umoleofsuc-AAFP-pNA/minuteat25°CinTris-HClpH8.0usingchymotrypsin.||AASequence:|MGSSHHHHHHSSGLVPRGSHMSVTLHTDVGDIKIEVFCERTPKTCENFLALCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKKFEDEYSEYLKHNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKTYRPLNDVHIKDITIHANPFAQ||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestablefor6months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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