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Usbio/301223 Anti-GPI, Recombinant, Human, His-Tag (Glucose-6-Phosphate Isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36)/100ug/301223
Usbio/301223 Anti-GPI, Recombinant, Human, His-Tag (Glucose-6-Phosphate Isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36)/100ug/301223
商品编号:
301223
品牌:
USbiological
市场价:
¥11160.00
美元价:
4743.00
产品分类:
其它重组蛋白
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.||Source:|Recombinant protein corresponding to aa1-558 and 578aa of human GPI, fused to His-tag at N-terminal expressed in E. coli.||Molecular Weight: |~65.3kD||Specific Activity: |>90units/mg ||Unit Definition:|One unit will convert 1.0umole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25ºC.||Endotoxin: |<1.0EU/1ug (LAL method)||AA Sequence:|MGSSHHHHHH SSGLVPRGSH MAALTRDPQF KLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK FCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT LEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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