Proteindisulfideisomerases(PDIs)constituteafamilyofstructurallyrelatedenzymeswhichcatalyzedisulfidebondsformation,reduction,orisomerizationofnewlysynthesizedproteinsinthelumenoftheendoplasmicreticulum(ER).Theyactalsoaschaperones,andare,therefore,partofaquality-controlsystemforthecorrectfoldingoftheproteinsinthesamesubcellularcompartment.PDIhasbeenfoundtohavemoderateeffects(25-fold)ontherateofoxidativefoldingofproteinsinvitro.RecombinantHumanProteinDisulfideIsomeraseisinvolvedindisulphide-bondformationandisomerization,aswellasthereductionofdisulphidebondsinproteins.RecombinantPDIhasbeenfoundtohavemoderateeffects(25-fold)ontherateofoxidativefoldingofproteinsinvitro.||Source:|RecombinantproteincorrespondingtohumanProteinDisulfideIsomerase,asingle,non-glycosylated,polypeptidechaincontaining508aa,fusedto6xHis-tagatN-terminal,expressedinE.coli.||MolecularWeight:|~57.12kD||ReductaseActivity:|~1.0x10e-3delta650nm/min-2.Bymeasuringtheturbidityincreaseat650nmduetoinsulinreduction(Holmgren,A.(1979)J.Biol.Chem.254,9627-9632).Theactivityisexpressedastheratiooftheslopeofalinearpartoftheturbiditycurvetothelagtime(Martnez-Galisteo,E.,PADIlla,C.A.,Garcia-Alfonso,C.,Lopez-Barea,J.,andBarcena,J.A.(1993)Biochimie(Paris)75,803–809).||IsomeraseActivity:|~0.5umolactiveRNaseAmin-1umolPDI-1.Accordingtothere-activationofreducedanddenaturedRNaseA(Lyles,M.M.andGilbert,H.F.(1991)Biochemistry30,613-619).||StorageandStABIlity:|Lyophilizedpowdermaybestoredat-20°C.ReconstitutewithsterileddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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