Malatedehydrogenase(EC1.1.1.37)isanenzymeinthecitricacidcyclethatcatalyzestheconversionofmalateintooxaloacetate(usingNAD+)andviceversa(thisisareversIBLereaction).Malatedehydrogenaseisnottobeconfusedwithmalicenzyme,whichcatalyzestheconversionofpyruvateusingNADPH.Malatedehydrogenaseisalsoinvolvedingluconeogenesis,thesynthesisofglucosefromsmallermolecules.Pyruvateinthemitochondriaisacteduponbypyruvatecarboxylasetoformoxaloacetate,acitricacidcycleintermediate.Inordertogettheoxaloacetateoutofthemitochondria,malatedehydrogenasereducesittomalate,anditthentraversestheinnermitochondrialmembrane.Onceinthecytosol,themalateisoxidizedbacktooxaloacetatebycytosolicmalatedehydrogenase.Finally,phosphoenol-pyruvatecarboxykinase(PEPCK)convertsoxaloacetatetophosphoenolpyruvate.||Source:|RecombinantproteincorrespondingtoMalatedehydrogenase,expressedinE.coli.TheDNAencodingMalate(Malic)DehydrogenaseisclonedfromCDNAlibraryofchickenheart.||UnitDefinition:|Oneunitisdefinedas1umolofNAD+production/minuteundertheassayconditions(25°C,pH7.5).||StorageandStABIlity:|Lyophilizedpowdermaybestoredat-20°C.ReconstitutewithsterileddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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