4000-520-616
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当前位置: 首页 > 产品中心 > Other_enzymes > Usbio/171977 Anti-PHGDH, Recombinant, Human, aa1-533 (Phosphoglycerate Dehydrogenase, PDG)/100ug/171977
商品详细Usbio/171977 Anti-PHGDH, Recombinant, Human, aa1-533 (Phosphoglycerate Dehydrogenase, PDG)/100ug/171977
Usbio/171977  Anti-PHGDH, Recombinant, Human, aa1-533 (Phosphoglycerate Dehydrogenase, PDG)/100ug/171977
Usbio/171977 Anti-PHGDH, Recombinant, Human, aa1-533 (Phosphoglycerate Dehydrogenase, PDG)/100ug/171977
商品编号: 171977
品牌: USbiological
市场价: ¥12100.00
美元价: 5142.50
产地: 美国(厂家直采)
公司:
产品分类: 其它酶
公司分类: Other_enzymes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Humanphosphoglyceratedehydrogenase(PGDH),alsoknownasPDG,amino-acids1–533(end)(GenBankAccessionNo.NM_006623)withC-terminalHistag,MW=57.5kD,expressedinanE.coliexpressionsystem.||Source:|Recombinantproteincorrespondingtoaa1-533fromhumanPHGDHatC-terminal,fusedtoHis-tagexpressedinE.coli.||MolecularWeight:|~57.5kD||SpecificActivity:|80pmol/min/ug||AssayConditions:|Thereactionmixturecontained100mMTris-HCl,pH8.8,400mMNaCl,0.2mMDTT,2.5mM3-phospho-D-glycericacid(SigmaP8877),1mMNAD,andvariousamountofPHGDHinafinalvolumeof50ul.ReactionswereinitiatedwiththeadditionofPHGDH,andtheincreaseinNADHfluorescence(Ex340/Em460nm)wascontinuouslymonitoredfor10minatroomtemperature.||Application:|Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestablefor6months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap
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