Control Peptide for C2069-54 Catenin, beta Pab RbxHu||b-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane|adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of b-Catenin|can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between a- and b-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of b-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of b-Catenin. This phosphorylation regulates b-Catenin binding to E-cadherin. Thus, sitespecific|tyrosine phosphorylation of b-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.||Applications: |Suitable for use in ELISA and for blocking antibody reactivity in Western Blot and Immunocytochemistry. Other applications not tested.||Recommended Dilution:|ELISA: 10-100ng/well. (Peptides were established in 96-well plates where the peptide was bound to plates for 2 hrs in 0.1 M sodium carbonate buffer, pH 8.5.)|Optimal dilution to be determined by the researcher||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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