Protein tags are protein or peptide sequences located either on the C-terminal or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc-Tag corresponds to amino acid residues (EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc-Tag. The antibody recognizes the Myc-Tag EQKLISEEDL fused to either the N-terminal or C-terminal of targeted proteins.||Applications: |Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence. Other applications not tested.||Recommended Dilution:|Western Blot: 1:2000-1:20,000|Immunoprecipitation: 1:2000-1:20,000|Immunofluorescence: 1:10-1:100|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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