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当前位置: 首页 > 产品中心 > Other_recombinant_protein > Usbio/219514 Anti-CAMK1D, Active, Recombinant, Human (Calcium/Calmodulin-Dependent Protein Kinase Type 1D)/5ug/219514
商品详细Usbio/219514 Anti-CAMK1D, Active, Recombinant, Human (Calcium/Calmodulin-Dependent Protein Kinase Type 1D)/5ug/219514
Usbio/219514  Anti-CAMK1D, Active, Recombinant, Human (Calcium/Calmodulin-Dependent Protein Kinase Type 1D)/5ug/219514
Usbio/219514 Anti-CAMK1D, Active, Recombinant, Human (Calcium/Calmodulin-Dependent Protein Kinase Type 1D)/5ug/219514
商品编号: 219514
品牌: USbiological
市场价: ¥10520.00
美元价: 4471.00
产地: 美国(厂家直采)
公司:
产品分类: 其它重组蛋白
公司分类: Other_recombinant_protein
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Calcium/calmodulin-dependent protein kinase ID (CAMK1D), or a novel Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK), showed kinase activity and that the activity was enhanced by Ca(2+) and calmodulin. Using a novel antibody generated against the C-terminus of CKLiK, CKLiK was detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes. Activation of human granulocytes by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF), but not the phorbol ester PMA (phorbol 12-myristate-13-acetate), resulted in induction of CKLiK activity, in parallel with a rise of intracellular [Ca2+]. Furthermore, fMLP-induced neutrophil migration on albumin-coated surfaces was perturbed, as well as beta2-integrin-mediated adhesion. These findings suggest a critical role for CKLiK in modulating chemoattractant-induced functional responses in human granulocytes (1). Also, CAMK1D exhibits Ca(2+)/CaM-dependent activity that is enhanced (~30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)alpha, consistent with detection of CAMK1D-activating activity in HeLa cells. Transiently expressed CAMK1D exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CAMK1D was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/CAMK1D cascade in HeLa cells.||Source:|Recombinant protein corresponding to active human CAMK1D, expressed in Baculovirus (Sf9 insect cells). ||Molecular Weight: |~60kD||Biological Activity:|~138nmol phosphate incorporated into Autocamtide 2/minute/mg protein at 30°C for 15 minutes using a final concentration of 50uM ATP (0.83uCi/assay).||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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