ControlpeptideforA3000-20(affinitypurifiedantibody)andA3000-21(antiserum).Syntheticpeptideconsistingof17aasequencemappingattheC-terminal,cytoplasmiclocation.NosignificanthomologyisseenwithotherAQPsoranyotherprotein.SpeciesSequenceHomology:Mouse:100%;Human:80%;Sheep76%.||Waterisacriticalcomponentofalllivingcells.Interestingly,tissuemembranesshowagreatdegreeofwaterpermeABIlity.Mammalianredcells,renalproximaltubules,anddescendingthinlimbofHenleareextraordinarilypermeabletowater.Watercrosseshydrophobicplasmamembraneseitherbysimplediffusionorthroughafacilitativetransportmechanismmediatedbyspecialprotein“aquaporin”.Overthelastdecade,genesforseveralmembersofaquaporinfamilyhavebeencloned,expressed,andtheirdistributionstudiedinmanytissues.Aquaporin-0orMIP26(majorintrinsicprotein26kD)andAquaporin-1(purifiedfromredcells)alsocalledCHIP-28(channelformingintegralprotein,28kD;268aa;genelocus7p14)hasbeenthefoundationofthegrowingfamilyofaquaporins.ThelensspecificAquaporin-0representsupto80%oftotallensmembraneprotein.DefectsinMIP26areacauseofautosomaldominantcataract.ThecataractFrasermutation(CAT-FRorShriveled)isatransposon-inducedsplicingerrorthatsubstitutesalongterminalrepeatsequencefortheC-terminusofMIP.Thelensopacitymutation(LOP)isanaasubstitutionthatinhibitstargetingofMIPtothecellmembrane.HumanAquaporin-0isa263aatransmembraneproteinbelongingtotheMIPfamily.Aquaporinfamiliesofproteinsarepredictedtocontainsixtransmembranedomains.TheNandC-terminusarepredictedtobecytoplasmic.||Applications:|SuitableforuseinELISAandAntibodyBlocking.NotsuitableforuesinWesternBlotduetolowmolecularweight.Otherapplicationsnottested.||RecommendedDilution:|ELISA:1ug/ml|AntibodyBlocking:5-10ugper1ugA3000-20(affinitypurifiedantibody)or1ulA3000-21|(antiserum).|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStability:|Maybestoredat4°Cforshort-termonly.Forlong-termstorage,storeat-20°C.Aliquotsarestableforatleast6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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