MEFcellsserveasfeedercellsthatsupportthegrowthofundifferentiatedmouseorhumanembryonicstemcells(ESCs)andinducedpluripotentstemcells(iPSCs).MEFcellsareisolatedfrom13.5-dayoldmouseembryosandshouldbeusedatearlypassages.Beforeuseasfeedercells,MEFcellsmustbemitoticallyinactivatedbyγ-irrADIationormitomycin-Ctreatment.|DR4MEFcellsarederivedfrommicethataregeneticallyengineeredwith4drug-resistantgenes,neomycin,hygromycin,puromycinand6-thioguanine.||Treatment:|gamma-irradiated||CellType:|MouseEmbryonicfibroblasts||StorageandStABIlity:|Forlong-termstorage,aliquottoavoidrepeatedfreezingandthawingandStoreinliquidnitrogen.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Aliquotsarestablefor6months.||Protocol:|1.Removeavialoffrozencellsfromliquidnitrogenandplaceitontodryicefor5’beforethawingitat37ºCwaterbath.Assoonasthemajorityofthecontentofthevialthawed,transferittoaconicaltubecontaining10xvolumeofpre-warmedmedium.||2.Spinat1000rpmfor5min,discardmedium,resUSPendthecellsingrowthmediumandplatethematanappropriatedensityinagelatin-coatedtissue-culturedish(generally25,000-50,000cells/cm2,AppendixIII).Optimaldensityistobedeterminedbytheuserforspecificapplications.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！