Usbio/353376-T25抗GFP表达的人视网膜微血管周细胞(T-25烧瓶)/T-25烧瓶/353376-T25
商品编号:
353376-T25
品牌:
USbiological
市场价:
¥22660.00
美元价:
9630.50
产品分类:
细胞分离
公司分类:
Cell_separation
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
PuromycinresistantGFP-HRMPswereselectedandshippedinproliferatingculturewith>90%confluence(cellsareprovidedatpassage3-5).PericytegrowthmediumcontainingFBSandgrowthsupplementisrecommendedforcellculture.Cellshaveanaveragepopulationdoublinglevelof>10whencultured.||Whenyoureceivethecells,leavetheflaskin37°CCO2incubatorfor1hour.Then,replacethetransportmediumwithfreshPericytegrowthmedium.Letthecellsgrowfor24hoursbeforesubculture.||Source:|GFPExpressingHumanRetinalMicrovascularPericytes,isolatedfromnormalhumanretinaltissueandtransfectedwithGFP-Lentiviralparticlesatpassage2.||CellNumber:|>90%confluentinT25flask||CellCharacterization:|CytoplasmicVWF/FactorVIII:<2%positivebyimmunofluorescence|CytoplasmicuptakeofDi-I-Ac-LDL:<2%positivebyimmunofluorescence|CytoplasmicAlpha-ActininFilaments:>80%positivebyimmunofluorescence|CytoplasmicDesminIntermediateFilaments:>80%positivebyimmunofluorescence||GFP-HRMPsarenegativeforHIV-1,HBV,HCV,andmycoplasma.||ImportantNote:|CellsareprovidedeitherinaT-25flaskorasa1mlfrozenvial.||Store1mlvialsat-70°C.StoreT-25flasksat37°Cin5%CO2.
品牌介绍
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