Usbio/353392-T25抗RFP表达的人脐静脉内皮细胞(T-25烧瓶)/T-25烧瓶/353392-T25
商品编号:
353392-T25
品牌:
USbiological
市场价:
¥17280.00
美元价:
7344.00
产品分类:
细胞分离
公司分类:
Cell_separation
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ZeocinresistantRFP-HUVECswereselectedandshippedinproliferatingculturewith>90%confluence(cellsareprovidedatpassage3).ENDO-Growthmediumcontaining5%serumandgrowthsupplementisrecommendedforculture.Cellshaveanaveragepopulationdoublinglevelof>18.||Whenyoureceivethecells,leavetheflaskin37°CCO2incubatorfor1hour.Then,replacethetransportmediumwithfreshENDO-Growthmedium.Letthecellsgrowfor24hoursbeforesubculture.||Source:|RFPExpressingHumanUmbilicalVeinEndothelialCells,isolatedfromnormalhumanumbilicalveinandtransfectedwithRFP-Lentiviralparticlesatpassage1.||CellNumber:|>90%confluent(>5x10e5cells)inT25flask||CellCharacterization:|CytoplasmicVWF/FactorVIII:>95%positivebyimmunofluorescence|CytoplasmicuptakeofDi-I-Ac-LDL:>95%positivebyimmunofluorescence|CytoplasmicPECAM1:>95%positivebyimmunofluorescence||RFP-HUVECsarenegativeforHIV-1,HBV,HCV,andmycoplasma.||ImportantNote:|CellsareprovidedeitherinaT-25flaskorasa1mlfrozenvial.||Store1mlvialsat-70°C.StoreT-25flasksat37°Cin5%CO2.
品牌介绍
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