Vitronectin is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhbits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. Studies show that anchorage dependent cells growing on ECM undergo more efficient plating, have a higher proliferation rate, reach a higher density, and require lower serum and growth factor concentrations—demonstrating enhanced differentiation potential.||Applications:|Suitable for use in ELISA and Cell Adhesion Assay. Other applications not tested.||Recommended Dilutions:|Cell Adhesion Assay: Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average|absorbance at 590nm of triplicate wells.|Optimal dilutions to be determined by the researcher. ||Storage and Stability:|Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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