Protein Western Blots are manufactured by using high-quality protein isolated from documented tissues. The tissue is excised, immediately frozen, and then pathologically identified. Protein is isolated by proprietary techniques. Each lane of the blot contains 50ug of protein.||The protein is separated by 4-20% gradient gel electrophoresis and transferred to a PVDF membrane. Human placenta protein is used as an interblot control lane for protein expression comparison between different blots.||Protein Western Blots are supplied ready for immediate use, so that you can immediately screen for your protein of interest without the hassle of obtaining hard to find tissues and processing the protein yourself.||Features:|Ready to use, pre-blotted with high quality protein|Suitable for both radioactive and non-radioactive probes|Extensive selection of blots|Documentation of tissues' clinical histories is available (additional cost)|“Matched" tissue slides or cDNA might be available for immunohistochemistry, in situ hybridization or PCR|High sensitivity and low background |Normalized based on amount of protein used in blots |Interblot control built into every blot||Applications:|Identification of normal, tumor or disease tissues specific gene expression|Protein size determination|Gene expression pattern analysis|Compare expression level of novel genes|Examining alternative splicing and prematur etermination of specific proteins||Tissues on Blots (different tissues, different single donors)|lane 1: heart|lane 2: brain|lane 3: kidney|lane 4: liver|lane 5: lung|lane 6: pancreas|lane 7: spleen|lane 8: skeletal muscle|lane 9: stomach|lane 10: small intestine|lane 11: colon|lane 12: rectum|lane 13: uterus|lane 14: prostate|lane 15: testis|lane 16: placenta||Storage Conditions:|Store blots at 4°C. Store moist in a sealed bag. Keep away from light.||Note: USBio recommends following protocol provided. Has not been evaluated for compatibility with other blocking and stripping methodologies or reagents.

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