Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture. It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity Transferrin has been identified also as one of the factors promoting the clonal growth of murine granulocyte and macrophage precursors cultured in vitro under serum-free conditions.||Source:|Human Serum||CAS Number:|11096-37-0||Appearance:|White to off-white powder||Endotoxin:|<1EU/mg||Solubility:|Readily soluble (10mg/mL in H2O)||Heat Treatment:|60°C for > 10 hours.||Iron Assay:|<30ug/g||Protein:|≥96% (Biuret)||Bioburden:|< 10CFU/mg||Molecular Weight:|~77kD||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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