Thrombospondin­4 (TSP-4) is an ~140kD matricellular protein that is secreted as a disulfide­linked pentamer. Within the Thrombospondin family, TSP­3 and TSP­5/COMP are also pentameric, while TSP­1 and TSP­2 are trimeric. TSP­4 regulates cell­cell and cell matrix interactions and plays a role in cardiovascular physiology and neuronal development (1, 2). Mature mouse TSP-4consists of an N­terminal heparin­binding domain, a coiled coil motif, four EGF­like repeats, seven TSP type­3 repeats (one with an RGD motif), and a TSP C­terminal domain (2, 3). Mouse TSP-4 shares 93% and 97% amino acid sequence identity with human and rat TSP-4, respectively. TSP-4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin­1, Fibronectin, and Matrilin­2 (4). Interactions of TSP-4 with non­collagenous proteins are independent of divalent cations, whereas interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP-4 binds to cell surface Integrins containing the alphaM, beta2, or beta3 chains (5, 6). It is expressed in skeletal muscle and tendon as well as by vascular smooth muscle and endothelial cells (7­9). It is up­regulated in cardiomyocytes during pressure overload and is required for mediating the responsive increase in cardiac contractility (10). In humans, a polymorphism of TSP­4 (A387P) is associated with myocardial infarction (11). TSP-4 contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature (5, 6). In the nervous system, TSP-4 is expressed by astrocytes and neurons and is enriched at neuromuscular junctions and synapse­rich layers of the brain and retina (9, 12, 13). It promotes neuronal adhesion, neurite outgrowth, and excitatory synaptogenesis (9, 13, 14). TSP-4 is up­regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia (12). It is also up­regulated in muscle following denervation (9). TSP-4 is additionally secreted by tumor­associated fibroblasts in gastric adenocarcinoma, particularly in regions of tumor cell invasion (15).||Description:|Recombinant protein corresponding to aa27-963, Gln27-Asn963, from mouse Thrombospondin-4, fused to 6xHis-Tag at C-terminal, expressed in CHO cells.||Molecular Weight:|~120-245kD||Endotoxin:|<0.1EU/1ug (LAL)||Biological Activity:|Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.5ug/ml.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！