ControlPeptideforM9555-01(serum)andM9555-01(affinitypurified).||EscherichiacoliMutYisanadenineDNAglycosylaseactiveonDNAsubstratescontainingA/G,A/8-oxoG,orA/CmismatchesandalsohasaweakguanineglycosylaseactivityonG/8-oxoG-containingDNA.TheN-terminaldomainofMutY,residues1-226,hasbeenshowntoretaincatalyticactivity.Thepost-replicativeadenine:8-oxo-7,8-dihydroguanine(GO)mismatchiscrucialforG:CtoT:Atransversion.ThismismatchiscorrectedbyEscherichiacoliMutYwhichexcisestheadeninefromA:GO.AcandidategenecodingforthehumancounterpartofMutYhasbeenclonedashMYH(535aa)(1)||Applications:|SuitableforuseinELISAorAntibodyBlocking.NotsuitableforuseinWesternBlotduetolowmolecularweight.Otherapplicationsnottested.||RecommendedDilutions:|AntibodyBlocking:5-10ugper1ulM9555-02(serum)orper1ugM9555-03(affinitypurifiedantibody).|ELISA:Coatplateswtih50-100ng/ml.|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Forlong-termstorage,aliquotandstoreat-20°C.Aliquotsarestableforatleast6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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