Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag- epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. GST, HA-tag, C-myc, and His-tag are the most commonly used tags for recombinant fusion proteins.|A specially designed recombinant protein contains 16-commonly used fusion protein tags.||Source:|Recombinant protein corresponding to 16-commonly used fusion protein tags, expressed in E. coli.||Recombinant protein (52kD) contains the following tags:|Trx|T7: M-A-S-M-T-G-G-Q-Q-M-G|HSV: QPE LAP EDP EDC|c-Myc: E-Q-K-L-I-S-E-E-D-L|VSV-G: Y-T-D-I-E-M-N-R-L-G-K|Glu-Glu: CEE EEY MPM E|V5: GKP IPN PLL GLD ST|E-tag: GAP VPY PDP LEP R|Flag: DYK DDD DK|S-tag: KET AAA KFE RQH MDS|HA-Tag: Y-P-Y-D-V-P-D-Y-A-C|KT3: KPP TPP PEP ET|E2: GVS STS SDF RDR|AU1: DTY RYI|AU5: TDF YLK |His-Tag: -H-H-H-H-H-H||Molecular Weight: |~52kD||Applications: |Suitable for use in Western Blot and ELISA. Other applications not tested.||Recommended Dilution:|ELISA: 1-10ul/ml for coating|Western Blot: load 1-5ul per lane for probing with most anti-tag antibodies|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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