Protein A (from Staphylococcus aureus, Cowan I Strain) is conjugated with highly purified horseradish peroxidase (RZ > 3.0). ||Applications:|Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. ||Recommended Dilutions:|Western Blot: 1:2000-1:10,000|Dot Blot: 1:2000-1:10,000|ELISA: 1:20,000-1:100,000|Immunohistochemistry: 1:1000-1:5000|Optimal dilutions to be determined by the researcher. ||Storage and Stability:|Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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