The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP pen. Each pen can be used to draw about 400 circles.||Applications: |Suitable for use in Immunohistochemistry and fluorescent staining methods. Other applications not tested.||Recommended Usage:|1) For Paraffin-embedded sections: |- Deparaffinize and rehydrate tissue sections.|- Rinse tissue sections with PBS and wipe off excess liquid around the tissue.|- Draw a circle on the glass slide around the section with Mini PAP pen.|- Dry the circle for 1-2 minutes at RT.|- Continue the normal immunohistostaining process.||2) For Frozen sections: |- Preclean glass slide.|- Coat slide with H5107: HistoGrip (50X concentrate) or other adhesive media (Optional).|- Cut and mount tissue sections on the slides.|- Rinse tissue sections with PBS and wipe off excess liquid around the tissue.|- Draw a circle on the glass slide around the section with Mini PAP pen.|- Dry the circle for 1-2 minutes at RT.|- Continue the normal immunohistostaining process.||Note: The water repellent circle is removable in xylene or xylene substitutes. Since it is not influenced by water soluble media, it will not interfere with microscopic study.||Storage and Stability:|Store at RT with cap on tight and tip pointed down. Press down on tip when pen starts to dry out.

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