The agarase is a recombinant protein from P. atlantica produced in E. coli. Agarase cleaves agarose to neoagaro-oligosaccharides, and is used for quantitative, gentle recovery of DNA from low melting point agarose.||Molecular Weight: |~36kD||Concentration: |2units/ul||Components:|219344: Agarase, beta, Recombinant, 1x500u Supplied as a liquid in 40mM Tris-HCl, pH 7.5, 50mM sodium chloride, 50% glycerol. ||219344A: Buffer, 10X, 1x2ml Supplied as a liquid 1M Tris-HCl pH 6.5, 10 mM EDTA||Unit Definition:|One unit is defined as the amount of enzyme required to digest 100ul (~100mg) of molten 1% low melting point agarose to neoagaro-oligosaccharides in 1 hour at 45°C.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||RECOVERY OF DNA FROM LOW MELTING POINT AGAROSE GEL:||1. Cut DNA band of interest from the low melting point agarose gel under a long-wave UV light, and transfer the agarose piece into a tared eppendorf tube.||2. Determine the weight of the agarose piece and add 0.1 volumes of 219344B: Buffer, 10X. Incubate the tube for 15 min at 65°C until the agarose is completely molten. A clear, nonviscous solution is required to be sure that all cleavage sites are accessible to the agarase.||3. Cool down the melten agarose to 42-45°C and add 1 unit 219344A: Agarase, beta, Recombinant per 100mg of agarose (100ul 1% agarose in Tris-acetate).||Note: When the percentage of agarose in higher than 1%, the units of agarase have to be adjusted in proportion. When using Tris-borate gel electrophoresis buffer, a two-fold amount of agarase should be used; otherwise,the incubation time has to be prolonged.||4. Carefully mix the solution and incubate for 1 hour at 42-45°C.||5. Add 0.1 volume of 3M sodium acetate, pH 5.5, to the melted agarose solution and incubate 15 min on ice||6. Centrifuge for 15 minutes at 4°C to pellet the oligosacchrides.||7. Precipitate the nucleic acids from the supernatant with 3 volume of ice-cold ethanol as usual.|||||||||

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！