Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.||Source:|Recombinant protein corresponding to aa1-187 from mouse DHFR, fused to His-tag at N-terminus, expressed in E. coli.||Molecular Weight: |~23.8kD (207aa, aa1-187 + NT His-Tag)||Biological Activity:|Specific activity is >0.2units/mg and was obtained by measuring the oxidation of NADPH in absorbance at 340nm during reaction. One unit will convert 1.0umole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP/minute at pH 6.5 at 25°C.||AA Sequence:|MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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