5'-Pu↓G A T C Py-3'|3'-Py C T A G↑Pu-5'||Source: Pseudomonas stutzeri Mck28-pH52||Concentration: |10u/ul||Unit Definition:|One unit is defined as the amount of PsuI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.||Form: |Supplied as a liquid in 10mM Tris-HCl, pH 7.4,100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.||Supplied with:|R1625: Restriction Enzyme Buffer A, 10X: |Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.||Supplied with R1625-01 Restriction Enzyme Buffer B, 10X: |Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2,0.1mg/ml BSA. ||Thermal Inactivation:|PsuI is inactivated by incubation at 80°C for 20 minutes.||Diluent Buffer:|10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mMDTT, 0.2mg/ml BSA and 50% glycerol||Overdigestion Assay:|No detectable change in the specific fragmentation pattern is observed after a 80-fold overdigestion with PsuI (5u/μg lambda DNA x 16 hours).||Ligation and Recleavage (L/R) Assay:|The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.||Labeled Oligonucleotide (LO) Assay:|No detectable degradation of single-stranded or double- stranded labeled oligonucleotides occurred during incubation with 10 units of PsuI for 4 hours.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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