5'-CA^TATG-3'|3'-GTAT^AC-5'||Buffers:|R1625-RestrictionEnzymeBufferA(doubledigests):1XBuffercomposition-Suppliedasaliquidcontaining33mMTris-acetatepH7.9,10mMmagnesiumacetate,66mMpotassiumacetate,0.1mg/mlBSA.Incubateat37°C.||R1625-03-RestrictionEnzymeBufferD(for100%digestion):1XBuffercomposition-Suppliedasaliquidin50mMTris-HCl,pH7.5,10mMMgCl2,100mMsodiumchloride,0.1mg/mlBSA.Incubateat37°C.||OverdigestionAssay:Nodetectablechangeinthespecificfragmentationpatternisobservedafter160-foldoverdigestion(10u/uglamBDaDNAx16hours)withNdeI.||Ligation/RecuttingAssay:After50-foldoverdigestion(3u/ugDNAx17hours)withNdeI,morethan95%oftheDNAfragmentscanbeligatedata5'-terminiconcentrationof0.1uM.Morethan95%ofthesecanberecut.||LabeledOligonucleotide(LO)Assay:Nodetectabledegradationofasingle-strandedanddouble-strandedlabeledoligonucleotidewasobservedafterincubationwith10unitsofrestrictionendonucleasefor4hours.||StABIlityduringProlongedIncubation:Aminimumof0.3unitsofNdelisrequiredforcompletedigestionof1ugoflambdaDNAin16hoursat37°C.||ThermalInactivation:Ndelisinactivatedbyincubationat65°Cfor20min.||DigestionofAgarose-embeddedDNA:Aminimumof5unitsofNdelisrequiredfordigestionof1ugofagarose-embeddedlambdaDNAin16hours.||CompatIBLeEnds:Csp6I,FspBI,Tru1I,VspI||NumberofRecognitionSitesinDNA:|Lambda:7|PhiX174:0|M13mp18/19:3|pBR322:1|pUC18/19:1|pUC57:1|pTZ19R/U:0||UnitDefinition:|OneunitisdefinedastheamountofNdelrequiredtodigest1ugoflambdaDNAin1hourat37°Cin50ulofassaybuffer.||DilutionBuffer:|10mMTris-HCl,pH7.4atRT,100mMKCl,1mMEDTA,1mMDTT,0.2mg/mlBSAand50%glycerol.||EnzymeActivityinBufferA(R1625)/BufferD(R1625-03):|R1625-0-20%|R1625(2X)-50-100%|R1625-03-100%

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