Tryptasebeta-1(MastCellProtease7)isaserineproteasewithtrypsin-likeactivity.Itisstoredinthesecretorygranulesofmousemastcells.ItexhibitsanticoagulantactivityduetoitsABIlitytodegradefibrinogeninthepresenceofthediversearrayofproteaseinhibitorsinplasma.Thededucedaminoacidsequenceconsistsofasignalpeptide(residues1-18),aproregion(residues19-28)andthematurechain(residue29-273).Thematurechainwasexpressedandpurified.||Source:|HumanCD33SignalPeptide(Met1-Ala16)MouseTryptasebeta-1(Ile29-Phe273).RecombinantmouseTyrptasebeta-1/MastCellProtease7(rmTryptasebeta-1,residues29-273)1intheaboveconstructwasexpressedinamurinemyelomacellline,NS0.||MolecularMass:Thepurified,secretedrecombinant,murineTryptasebeta-1correspondstothematureformwiththeN-terminalsequenceofI29VGGQEAHGN.BySDS-PAGE,theproteinmigratesasthedoubletwithapparentmolecularmassesofapproximately37and36kD.||Activity:Measuredbyitsabilitytocleaveapeptidesubstrate,Benzyloxcarbonyl-Lys-Thiobenzylester(Z-K-SBzl),inthepresenceof5,5’-Dithio-bis(1-nitrobenzoicacid)(DTNB),asmeasuredby|absorbanceat405nmandusingtheextinctioncoefficientof13,260M-1cm-1.2.Thespecificactivity,measuredwith100uMZ-K-SBzland500ngenzymein100ulof50mMMES,pH6.5containing0.1mMDTNBatRT,is≥200pmoles/min/ug.||Storage:Stableforuptotwelvemonthsat-70°Cfromthedateofreceipt.Theproteincanbealiquotedandstoredat-20°to-70°Cinamanualdefrostfreezerforsixmonthswithoutasignificantlossofactivity.Avoidrepeatedfreeze-thawcycles.

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