GlyoxalaseI,alsoknownasGLO1,belongstotheglyoxalaseⅠfamily.GlyoxalaseIisresponsIBLeforthecatalysisandformationofS-lactoyl-glutathionefrommethylglyoxalcondensationandreducedglutathione.Thisenzymeisubiquitouslyexpressedandisalsopresentinmanytumorcelllines,inwhichitsconcentrationisoftenupregulated.RecombinanthumanGLO1proteinwasexpressedinE.coliandpurifiedbyusingconventionalchromatographytechniques.||AASequence:|MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIA|WALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKM|ATLM||EnzymeActivity:|Notdetermined.Thisproductisrecommendedforuseinapplicationsthatdonotrequireacatalyticallyactiveformoftheprotein.||StorageandStABIlity:|Forlong-termstorage,aliquotandstoreat-20°C.Aliquotsarestableforatleast6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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