Tryptophan2,3-dioxygenase(TDO)isaheme-containingdioxygenasecatalyzingtheadditionofmolecularoxygenacrossthe2,3-doublebondoftheindoleringoftryptophantoformN-formylkynurenine(NFK).InAnophelesgambiae,TDOistheonlyenzymeabletocatalyzethefirstandrate-limitingstepinL-Trpcatabolismthroughthekynureninepathway.Tryptophanisanessentialaminoacidforproteinsynthesisandalsotheprecursorforproductionofanumberofneurotransmitters,suchasSEROtoninandmelatonin;inmosquitoes,thekynureninepathwayisessentialforeyepigmentation.Conceivably,thetryptophan-usingpathwaysshouldberegulatedinacoordinatedmannerinmosquitoesaswellasinotherspeciesandTDOactivation/inactivationprocessescouldplayanessentialroleinthesephenomena.||Source:|Recombinantproteincorrespondingtofull-lengthAnophelesgambiaeTDO,fusedtoHis-tagatN-terminal,expressedinE.coli.||MolecularWeight:|~47.9kD||Applications:|Canbeusedtotestpotentialkynureninepathwayinhibitorsinthemosquito,bycontinuouslymonitoringtherateofNFKformationat321nm(E=3152/Mcm)ininvitrospectrophotometricassays.||BIOLOGicalActivity:|SpecificactivitytowardsL-Trp:87.0umol/hrpermg.TDOcatalyzestheoxidativeopeningoftheL-tryptophanindolemoietytoyieldN-formylkynurenine(NFK).||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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