DeoxyribonucleaseI(rhDNase),isanenzymewhichselectivelycleavesDNA.RecombinantHumanDnaseisanendonucleaseenzymewhichsplitsphosphodiesterlinkageswithinpolynucleotides,actingprimarelyonsinglestrandedDNA(ssDNA),doublestrandedDNA(ddDNA)andchromatin.DnaseisactivatedbybivalentmetalssuchasMg+2andCa+2.||DnaseenzymesarecommonreagentsusedinbiochemicalmethodsrequiringdiestionofDNAandrecoveryofRNA,orwhereDNAistoberemovedwithoutaffectingstructuralproteinsorenzymes.DnaseenzymesarealsousedintissueculturetodigestDNAfromdamagedcells,resultinginreducedviscosity,andforremovalofmembrane-boundDNAfragments.||Source:|Recombinantproteincorrespondingto260aafromhumanDeoxyribonucleaseI,expressedinCHOcells.||MolecularWeight:|~37kD||UnitDefinition:|OneDnaseunitresultsinanincreaseinabsorbanceat260nmof0.001/minuteat25CwhenactinguponhighlypolymerizedsolutionofDNAatpH-5.Also0.005Kunitzunitdigests1ugoflamBDaphageDNAin10minutesat37Cin50mMTris,1mMMg++,pH7.8ina50ulreaction.||Applications:|SuitableforuseinELISAandWesternBlot.Otherapplicationsnottested.||RecommendedDilution:|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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