Exonuclease III is a 3’-5’ exonuclease releasing 5’ mononucleotides from dsDNA and exhibits AP-specific endonuclease, 3’ phosphatase and RNAse H activities.||Source:|E.coli cells carrying a cloned E.coli xth gene.||Storage Buffer:|Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM KCl, 1mM DTT, 50% glycerol.||Reaction Buffer (10X) E9008-05:|Supplied as a liquid in 660mM Tris-HCl, pH 8.0, 6.6mM MgCl2||Unit Definition:|One unit of Exonuclease III catalyzes the release of 1nmol of acid soluble reaction products from E. coli [3H]-DNA in 30 min at 37°C.||Applications:|Suitable for use in construction of nested unidirectional deletions of DNA fragments,generation of single-stranded template for DNA dideoxy sequencing, site-directed mutagenesis, cloning of PCR products and preparation of strand-specific probes.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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