Ribonuclease H specifically degrades only the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded or double-stranded DNA and RNA.||Unit Definition:|One unit catalyzes the formation of 1nmol of acid soluble products in 20min at 37ºC. Enzyme activity is assayed in the following mixture: 20mM Tris-HCl (H 7.8), 40 mM KCl, 8mM MgCl2, 1mM DTT, 24uM [3H]-POLY(A)-POLY(dT), 0.03mg/ml BSA AND 4% Glycerol.||10X Reaction buffer (included):|R2021A|Supplied as a liquid in 200mM Tris HCl, pH 8.0, 400mM KCl, 80mM MgCl2, 10mM DTT.||Applications:|Suitable for use in Removal of mRNA prior to the synthesis of the 2nd strand of cDNA, Removal of mRNA poly(A) sequences after hybridization with oligo(dT), Site-specific cleavage of RNA and Studies of in vitro polyadenylation rection products.||Inhibition and Inactivation:|Inhibitors: metal chelators, SH-blocking reagents.|-Inactivated by heating at 65ºC for 10min.||Endodeoxyribonuclease Assay:|No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of10 units of Ribonuclease H with 1ug of pBR322 DNA|in 50ul of reaction buffer for 1 hour at 37°C. ||Exodeoxyribonuclease Assay:|0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubationof 10 units of Ribonuclease H with 1ug of sonicated|E.coli [3H]-DNA in 50ul of reaction buffer for 1 hour at 37°C. ||Ribonuclease Assay:|0% of the total radioactivity was released into trichloroacetic acid-soluble fraction after incubation of10 units of Ribonuclease H with 1ug of [3H]-RNA in 50ul of reaction buffer for 1 hour at 37°C.||Storage and Stability:|For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。 恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！