4000-520-616
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主营:主营:抗体,重组蛋白,细胞培养基和分子生物学试剂盒
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4000-520-616
当前位置: 首页 > 产品中心 > culture_medium > Usbio/M2856-01 Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
商品详细Usbio/M2856-01 Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
Usbio/M2856-01  Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
Usbio/M2856-01 Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
商品编号: M2856-01
品牌: USbiological
市场价: ¥10620.00
美元价: 4513.50
产地: 美国(厂家直采)
公司:
产品分类: 培养基
公司分类: culture_medium
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
In1950,Morgan,MortonandParkerdescribedoneofthefirsttotallydefinedmediathatdidnotdependlargelyonanimalproductsorextractsasnutritivesources.Thismediumsupportedthegrowthofprimarychickembryoheartcells.IthassincebecomeknownasMedium199.Itscurrentuseiswiththeadditionofseruminvirusandvaccineproduction,andinthecultureofmanynon-transformedcells.Itisalsousedincombinationwithlesscomplexmedia.Medium199modifiedwithEarle'sBalancedSaltsisdesignedforusewithcellsmaintainedinambient(non-CO2)atmosphericconditions.M2852differsfromtheoriginalformulationbycontainingallL-aminoacids.Theoriginalformulationcontained10DL-aminoacids,whicharelessBIOLOGicallyactiveonapergrambasisthanthecorrespondingL-aminoacids.||Thesearchforasyntheticmediumtoreplaceserumformaintainingcellsinvitrobeganinthelatenineteenthcenturyandcontinuestothisday.Ringer,LocksandTyrodesubstitutedphysiologicalsaltsolutionsaugmentedwithglucoseforserumandtherebylaidthefoundationforthedevelopmentofdefinedmedia.Asbiochemicalandanalyticaltechniqueshaveimproved,moreofthecomponentsinserumsuchasvitamins,hormones,andaminoacidshavebeenidentifiedandincorporatedintophysiologicalsaltsolutions,reducing,andinsomecaseseliminatingtheconcentrationsofanimalserarequiredasamediumsupplement.Althoughtherehavebeenmanymodificationstotheoriginalformulasineffortstoproducefullydefinedmedia,saltsolutionsstillplayanimportantroleintissueculture.Asaltsolution'sbasicfunction,tomaintainthepHandosmoticbalanceinthemediumandtoprovidethecellswithwaterandessentialinorganicions,isasvaluabletodayaswhenitwasfirstdevelopedacenturyago.Earle’sBalancedSaltsselectionisstronglyinfluencedby1.typeofcell,2.typeofculturemonolayer,sUSPension,clonal,and3.degreeofchemicaldefinitionnecessary.Itisimportanttoreviewtheliteratureforrecommendationsconcerningmedium,supplementationandphysiologicalparametersrequiredforaspecificcellline.
品牌介绍
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