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Usbio/M2856-01 Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
Usbio/M2856-01 Anti-Medium 199 w/ Hanks’ w/o L-Glutamine, Phenol Red (Powder, TC 199)/25L/M2856-01
商品编号:
M2856-01
品牌:
USbiological
市场价:
¥10620.00
美元价:
4513.50
产品分类:
培养基
公司分类:
culture_medium
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
In1950,Morgan,MortonandParkerdescribedoneofthefirsttotallydefinedmediathatdidnotdependlargelyonanimalproductsorextractsasnutritivesources.Thismediumsupportedthegrowthofprimarychickembryoheartcells.IthassincebecomeknownasMedium199.Itscurrentuseiswiththeadditionofseruminvirusandvaccineproduction,andinthecultureofmanynon-transformedcells.Itisalsousedincombinationwithlesscomplexmedia.Medium199modifiedwithEarle'sBalancedSaltsisdesignedforusewithcellsmaintainedinambient(non-CO2)atmosphericconditions.M2852differsfromtheoriginalformulationbycontainingallL-aminoacids.Theoriginalformulationcontained10DL-aminoacids,whicharelessBIOLOGicallyactiveonapergrambasisthanthecorrespondingL-aminoacids.||Thesearchforasyntheticmediumtoreplaceserumformaintainingcellsinvitrobeganinthelatenineteenthcenturyandcontinuestothisday.Ringer,LocksandTyrodesubstitutedphysiologicalsaltsolutionsaugmentedwithglucoseforserumandtherebylaidthefoundationforthedevelopmentofdefinedmedia.Asbiochemicalandanalyticaltechniqueshaveimproved,moreofthecomponentsinserumsuchasvitamins,hormones,andaminoacidshavebeenidentifiedandincorporatedintophysiologicalsaltsolutions,reducing,andinsomecaseseliminatingtheconcentrationsofanimalserarequiredasamediumsupplement.Althoughtherehavebeenmanymodificationstotheoriginalformulasineffortstoproducefullydefinedmedia,saltsolutionsstillplayanimportantroleintissueculture.Asaltsolution'sbasicfunction,tomaintainthepHandosmoticbalanceinthemediumandtoprovidethecellswithwaterandessentialinorganicions,isasvaluabletodayaswhenitwasfirstdevelopedacenturyago.Earle’sBalancedSaltsselectionisstronglyinfluencedby1.typeofcell,2.typeofculturemonolayer,sUSPension,clonal,and3.degreeofchemicaldefinitionnecessary.Itisimportanttoreviewtheliteratureforrecommendationsconcerningmedium,supplementationandphysiologicalparametersrequiredforaspecificcellline.
品牌介绍
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