Description:|MouseMSCfreezingmediumwasoptimizedforcryopreservationofourMousemesenchymalstemcells(mMSC).|Thisproductcontainsfetalbovineserum.Eachlotistestedforsterility.||ProcedureforFreezingmMSC:|1.FreezemouseMSCcellswhentheyreach>95%confluence.|2.WarmDulbecco’sPBS(withoutCa2+andMg2+bath.ThawmMSCfreezingmediumatroomtemperature.)and0.05%trypspin/EDTAina37°Cwater|3.RemovemMSCgrowthmedium,rinsemMSCwithDulbecco’sPBS,andincubatethecellswithtrypsin/EDTAsolutionuntilapproximately90%ofthecellsbegintodetach.Monitorthecellsunderamicroscopetoavoidover-trypsinization.|4.Addfetalbovineserumequalto1/10volumeofthetrypsin/EDTAsolutiontoneutralizetrypsin.Gentlyshaketheculturevesseltomix.|5.TransferthecellsUSPensionintoa15mlconicaltube.Takeasmallaliquotofthesuspensiontocountcells.Centrifugethecellsuspension250xgfor5minutesatroomtemperature.|6.Carefullyremovethesupernatant.AddavolumeofmMSCfreezingmediumandresuspendcellsto1x10e6perml.Dispense1mlintoeachcryovial.|7.Placecryovialsinafreezingcontainertoslowlylowerthetemperatureina-80°Cfreezer.Transferthevialstoliquidnitrogenthenextdayforlongtermstorage.||StorageandStABIlity:|Storeat-20°C.Stablefor1yearfromthedateofreceiptunderproperstoragecondition.

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