EPOCgrowthmediumwasdesignedandtestedforhumanendotheliumProgenitorOutgrowthcells(EPOC).Thismediumincludestwocomponents:EPOCbasalmedium(E3010-68G1)andEPOCgrowthmediumsupplement(E3010-68G2).TheEPCgrowthmediumsupplementincludesfetalbovineserumandhumanrecombinantgrowthfactors,andsomelot-to-lotvariABIlityisunavoidable.||Eachlotofthisproductistestedincellculturetoensureitssterility.Thisproductdoesnotcontainantibiotics.||KitComponents:|E3010-68G1:EndothelialProgenitorOutgrowthCells,Human,BasalMedium||E3010-68G2:EndothelialProgenitorOutgrowthCells,Human,GrowthMediumSupplement||UseofEPOCGrowthMediumforCulturingEPOC:|1.ThawtheE3010-68G2andaddtheentirevolume(25ml)totheE3010-68G1(500ml).Antibioticsmayalsobeaddedifcontaminationisaconcern.|2.TaketheamountofEPOCgrowthmediumneededandwarmto37°Cinawaterbathorincubator.Avoidfrequenttemperaturechangetotheentirebottleofmedium.TheEPOCgrowthmediumisstablefor1monthstoredat4°C.|3.RefertomanualforEPOCcultureproceduresusingtheEPOCgrowthmedium.Werecommendchangingmediumevery2daysforregularcellculture..||StorageandStability:|TheEPOCbasalmediumisstoredat4°C.Achangeincolororappearanceofprecipitatemayindicatedeterioration.TheEPOCgrowthmediumsupplementisstoredat-20°C.Iftakingaliquotsfromtheoriginalbottleisnecessary,dispensethecontentsandre-freezeinsmallaliquotstoavoidrepeatedfreezing-thawingcycles.EPOCbasalmedium:Stablefor6monthsfromthedateofreceiptunderproperstoragecondition.EPOCgrowthmediumsupplement:Stablefor6monthsfromthedateofreceiptunderproperstoragecondition.

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