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Usbio/E3010-68F Anti-Endothelial Progenitor Outgrowth Cells, Human, Freezing Medium/50ml/E3010-68F
Usbio/E3010-68F Anti-Endothelial Progenitor Outgrowth Cells, Human, Freezing Medium/50ml/E3010-68F
商品编号：
E3010-68F
品牌：
USbiological
市场价：
¥6540.00
美元价：
2779.50
产品分类：
培养基
公司分类：
culture_medium
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:|EPOCfreezingmediumwasoptimizedforcryopreservationofourhumanendotheliumProgenitorOutgrowthcells(EPOC).|Thisproductcontainsfetalbovineserum.Eachlotistestedforsterility.||ProcedureforFreezingEPOC:|1.FreezeEPCcellswhentheyreach90%to95%confluence.|2.WarmDulbecco’sPBS(withoutCa2+andMg2+)and0.05%trypspin/EDTAina37°Cwaterbath.ThawEPOCfreezingmediumatroomtemperature.|3.RemoveEPOCgrowthmedium,rinseEPOCwithDulbecco’sPBS,andincubatethecellswithtrypsin/EDTAsolutionuntilapproximately90%ofthecellsbegintodetach.Monitorthecellsunderamicroscopetoavoidover-trypsinization.|4.Addfetalbovineserumequalto1/10volumeofthetrypsin/EDTAsolutiontoneutralizetrypsin.Gentlyshaketheculturevesseltomix.|5.TransferthecellsUSPensionintoa15mlconicaltube.Takeasmallaliquotofthesuspensiontocountcells.Centrifugethecellsuspension150xgfor5minutesatroomtemperature.|6.Carefullyremovethesupernatant.AddavolumeofEPOCfreezingmediumandre-suspendcellsto0.5to1x10e6perml.Dispense1mlintoeachcryovial.|7.Placecryovialsinafreezingcontainertoslowlylowerthetemperatureina-80°Cfreezer.Transferthevialstoliquidnitrogenthenextdayforlongtermstorage.||StorageandStABIlity:|Storeat-20°CStablefor1yearfromthedateofreceiptunderproperstoragecondition.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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