CD133+humanEPCfreezingmediumwasoptimizedforcryopreservationofourhumanendotheliumProgenitorcells(hEPC).||Thisproductcontainsfetalbovineserum.Eachlotistestedforsterility.||ProcedureforFreezingEPC:|1.WarmDulbecco’sPBS(withoutCa2+andMg2+)ina37°Cwaterbath.ThawEPOCfreezingmediumatRT.||2.Gentlyshaketheculturevesseltomix.Takeasmallaliquotofthesupensiontocountcells.||3.TransferthecellsUSPensionintoaconicaltube.||4.RinseEPCculturevesselwithDulbecco’sPBSandtransfertotheconicaltubewithpreviouscellsuspension.||5.Centrifugethetubeat150xgfor5minutesatRT.||6.Carefullyremovethesupernatantwithoutdisturbingthepellet.||7.AddtheappropriatevolumeofE3010-60Fandresuspendcellsto0.5to1x10e6/ml.Dispense1mlintoeachcryovial.||8.Placecryovialsinafreezingcontainertoslowlylowerthetemperatureina-80ºCfreezer.||9.Transferthevialstoliquidnitrogenthenextdayforlongtermstorage.||StorageandStABIlity:|Storeat-20°C.Aliquotsarestablefor6monthsafterreceiptat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

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