CryopreservationMedia(BRFFGTC-80,FreezingMediaPair)isusedtocryopreservesUSPensionsofviablecellsdetachedfrommonolayerculturesusingthe"coldtrypsin"methodwithPET(catalog#P3357).CellswhichhavebeencryopreservedinthismediahavebeenshowntohavesuperiorviABIlityuponreconstitution.TheFreezingMediaPairhasbeenusedextensivelytocryopreservehumancellsgrowninserum-freemedia.||SuggestedUse:|Tocryopreservecells,centrifugethecellsuspension.Resuspendthecellsat4x10e6cells/mlinice-coldSolutionA.Slowlyaddanequalvolumeofice-coldSolutionBtothecellsuspension.Mixwell.Transfer1mlaliquotsofthecellsuspensiontopre-cooledcryovials.Freezeslowlyusinganautomaticcontrolledrate(1°Cperminute)freezerto-90°C.Alternatively,placethecryovialsinsideaStyrofoamrackandplacetherackina-80°Cmechanicalfreezerfor24hours.Transferthefrozenvialstoaliquidnitrogenfreezer(vaporphase)forpermanentstorage.||Kitcomponents:|C7938ASolutionA2x25ml|C7938BSolutionB2x25ml||StorageandStability:|Storeat-70°C.Stableforatleast12months.|Stabilityat4°C:3months|pH:7.7-7.9fora1:1mixtureofSolutionAandSolutionB.

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