CellAttachmentReagent(FNCCoatingMix,BRFFAF-10)isformulatedtodramaticallyincreasetheattachmentofcellstotheplasticgrowthsubstratumusedforculturingmammaliancelllines.Manyfastidiouscelltypessuchashumanprostateandbreastepithelialcellsplatedintheappropriateserum-freemediumwillattachandgrowmoreeffectivelyonFNC-coatedplasticsurfaces.||CellAttachmentReagentcanincreasethesurvivalrateofcelllinesfrom5%to95%duringthecellgrowthtransferprocess.Labresearcherstypicallyusesmallflasksorglassplatestogrowcelllines,thentransferthemtwotimestolargersurfacesasthecellsproliferate.TrADItionallyonly5%ofthecelllinessurviveeachtimetheyaretransferred.TheCellAttachmentReagentcoatstheflasksandplateswithapolymerthathelpsthecellsattachfaster,avertingthehighrateofcelldeath.Mostcelltypessuchashumanprostate,breastandepithelial(skin)cellswillattachandgrowwellonFNC-coatedsurfaces.||pH:|7.4±0.2||Color:|LighttomediumpinkduetopHindicator.Colorhasnoeffectuponperformanceofproduct.||Conductivity:|13-20mS||SterilityTest:|Nodetectablemicrobialgrowth.||FunctionalTest:|>50%cellattachmenttocoatedplasticsubstratumvsuncoatedsubstratium.||RecommendedUsage:|Tocoatthegrowthsurface,add0.2ml/cm2ofsurfaceareaoftheculturevessel.Forexample,use5mlforaT-25flask.Thesurfaceshouldbecompletelycoveredwithalayerofliquid.Incubatefor30seconds.RemovetheCellAttachmentReagentwithapipet.ResidualCellAttachmentReagentwillnotadverselyaffecttheviABIlityofthecellstobeplated.Donotreturnany“used”reagenttotheoriginalcontainer.Thiswillcompromisetheperformanceoftheproduct.Thecoatingmaybeperformedimmediatelyoruptoonehourpriortoseedingthecells.||StorageandStability:|Storeat4°C.DoNotFreeze.Stablefor6months.||Note:ColorislighttomediumpinkduetopresenceofpHindicator.Variationsincolorhasnoeffectuponperformanceofproduct.Colorfadeswithage.

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