Digoxigeninisconsideredahapten,asmallmoleculewithhighimmunigenicityusedinmanymolecularBIOLOGyapplications.OtherpopularhaptensincludeDNP(dinitrophenol),biotinandfluorescein.Ingeneral,antibodiesgeneratedagainsthaptenshavehigheraffinitiesfortheirtargetsthanotherantibodies,sohaptensareconjugatedtootherbiologicalmoleculesasall-purposeimmuno-tags.Thisisbecauseanti-haptenantibodieshavehighaffinitiesandarereADIlyavailable,whileantibodiesmaynotbecommerciallyavailableforexoticbiologicaltargets.DigoxigeninisastandardimmunohistochemicalMarkerforinsituhybridization.InthiscaseitisconjugatedtoasinglespeciesofRNAnucleotidetriphosphate(typicallyUridine),whichisthenincorporatedintoRNA(a"riboprobe")asitissynthesizedbythecellularmachinery.Later,theDigoxigenin("Dig")markersaredetectedwithanti-digoxigeninantibodies,whichmaybelabeledwithdyesorenzymes(seeELISA)forvisualizationanddetection.Inasimilarmanner,Digoxigeninmaybeconjugatedtosugarstostudyglycosylationeventsinbiologicalsystems.