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Usbio/028239 Surfactant Associated Protein A (SPA) BioAssay™ ELISA Kit (Mouse)/028239/96Tests
Usbio/028239 Surfactant Associated Protein A (SPA) BioAssay™ ELISA Kit (Mouse)/028239/96Tests
商品编号：
028239
品牌：
USbiological
市场价：
¥16480.00
美元价：
7004.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Surfactant Associated Protein A (SPA) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SPA in mouse serum, plasma, lung lavage fluid and other biological fluids.
Detection Range
31.2-2000pg/ml
Sensitivity
14.1pg/ml
Intra-Assay CV
<10%
Inter-Assay CV
<12%
Kit Components
*028239A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*028239B: Standard, 2x1vial028239C: Standard Diluent, 1x10ml or 1x20ml*028239D: Detection Reagent A (green), 1x60ul or 1x120ul*028239E: Detection Reagent B (red), 1x60ul or 1x120ul028239F: Assay Diluent A, 1x6ml or 1x12ml028239G: Assay Diluent B, 1x6ml or 1x12ml028239H: TMB Substrate, 1x4.5ml or 1x9ml*028239J: Positive Control, 735.2pg/ml, 1x1vial (Lyophilized)028239K: Stop Solution, 1x3ml or 1x6ml028239L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution
The Stop Solution (028239K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *028239A, *028239B, *028239D, *028239E and *028239J at -20°C. After reconstitution of *028239J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 028239J: Positive Control
Intended Use
The positive control is from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics
The positive control vial contains mouse SPA in mouse serum (lyophilized powder). Note: The mouse source material used in this product are from healthy animal of SPF standard (specific pathogen-free animals).
Expected Values
506.2pg/ml
Usage: |Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary
1. Prepare all reagents, samples and standards;2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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