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Usbio/028707 Tumor Protein P53 (TP53) BioAssay™ ELISA Kit (Human)/028707/96Tests
Usbio/028707 Tumor Protein P53 (TP53) BioAssay™ ELISA Kit (Human)/028707/96Tests
商品编号：
028707
品牌：
USbiological
市场价：
¥16180.00
美元价：
6876.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The Human Tumor Protein p53 (TP53) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TP53 in human serum, plasma and other biological fluids.
Detection Range
78-5,000pg/ml
Sensitivity
28pg/ml
Precision
Intra-Assay: CV<10%Inter-Assay: CV<12%
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TP53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TP53 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
*028707A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*028707B: Standard, 2x1vial 028707C: Standard Diluent, 1x20ml*028707D: Detection Reagent A, 1x120ul *028707E: Detection Reagent B, 1x120ul 028707F: Assay Diluent A, 1x12ml028707G: Assay Diluent B, 1x12ml028707H: TMB Substrate, 1x9ml028707K: Stop Solution, 1x6ml028707L: Wash Buffer, 30x, 1x20ml
Storage and Stability
Store *028707A, *028707B, *028707D and *028707E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
品牌介绍
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