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Usbio/351635 Epidermal Growth Factor (EGF) BioAssay™ ELISA Kit (Human)/351635/48Tests
Usbio/351635 Epidermal Growth Factor (EGF) BioAssay™ ELISA Kit (Human)/351635/48Tests
商品编号：
351635
品牌：
USbiological
市场价：
¥13020.00
美元价：
5533.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Sample Type
Serum, plasma, cell culture supernates, tissue homogenates
Intended Use
For the quantitative determination of human epidermal growth factor (EGF) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
Sensitivity
0.39pg/ml
Range
1.56-100pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of human EGF. No significant cross-reactivity or interference between human EGF and analogues was observed.
Test Principle
This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate (12x8 coated microwells), 1x96 wellsStandard (Freeze dried), 2x vialsBiotin-antibody (100X), 1x120ulHRP-avidin (100X), 1x120ulBiotin-antibody Diluent, 1x15mlHRP-avidin Diluent, 1x15mlSample Diluent, 1x50mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manual
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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